Mutation Detection : A Practical Approach

Mutation Detection : A Practical Approach

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Mutation detection is increasingly undertaken in a wide spectrum of research areas: in medicine it is fundamental in isolating disease genes and diagnbosis, and is especially important in cancer research; in biology, commercially important genes can be identified by the mutations they contain. But mutation detection is time-consuming and expensive. This volume offers the latest tried and tested protocols for a range of detection methods, from the labs of the leading researchers in the field. - ;Mutation detection is increasingly undertaken in a wide spectrum of research areas: in medicine it is fundamental in isolating disease genes and diagnosis, and is especially important in cancer research; in biology, commercially important genes can be identified by the mutations they contain. But mutation detection is time-consuming and expensive. This volume offers the latest tried and tested protocols for a range of detection methods, from the labs of the leading researchers in the field. Contents: Single strand conformation polymorphism analysis; Single-stranded conformation polymorphism and heteroduplex analysis; Comprehensive mutation detection with denaturing gradient gel electrophoresis; Cleavage using RNase to detect mutations; Cleavage of mismatched bases using chemical reagents; Mutation detection using T4 endonuclease VII; Detection of mutations by hybridization with sequence-specific oligonucleotide probes; DNA detection and sequence distinction through oligonucleotide ligation; Detection of sequence variation using primer extension; Selective amplification of specific alleles; The protein truncation text (PTT); Functional assay of the p53 tumour suppressor gene; Advances in direct DNA sequencing for mutation scanning; Appendix: List of Suppliers; Index. -Fresh tumour tissues are snap-frozen for subsequent manual microdissection under the microscope. Tumour nests containing less than 50 cells can be isolated from 16 |xm cry os tat sections stained with methylene blue and/or p53- specificanbsp;...


Title:Mutation Detection : A Practical Approach
Author: R. G. H. Cotton, E. Edkins, S. Forrest
Publisher:Oxford University Press, UK - 1998-02-05
ISBN-13:

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